Tritium-labeled compounds V. Radioassay of both carbon-14 and tritium in films, with a proportional counter
نویسندگان
چکیده
منابع مشابه
Synthesis of carbon-14 and tritium labeled sagamicin.
Sagamicin, an aminoglycoside antibiotic, was labeled with tritium by means of a platinum catalyzed hydrogen exchange reaction and by carbon-14 in two biosynthetic procedures. 14C-Methyl-L-methionine was used as the radioactive precursor in these biological processes involving Micromonospora sagamiensis. The distribution of radioactivity in 14C-sagamicin and gentamicin C1 was studied by mild aci...
متن کاملTritium - Labeled Compounds III . Aldoses - i - ( , 2
In a n c,," method fo r prepar ing aldoses labeled wit h Lritium at ca r bon l , Lhc aldoni c lactone l S rcdu ced with li thium boro.hyddde-I in p yridine soluLion . The mcthod is suitablc for prcparing aldosesl -1 h a vin g high specifi c act ivit ies. Thc crudc product con tains a substant ia l proport ion of t hc c<?lTespondin g a ldi tol-l-I, but t he purc aldosc-l-1 is rcadil y scparated ...
متن کاملTritium - Labeled Compounds II . General - Purpose Appar - and Procedures Use of Tritium atus , and Lithium for the Preparation , Analysis , Oxide and Tritium - Labeled Borohydride
A general-purpose manifold is descd bed, which is useful for numerous proced ures involving trit ium gas and tritium-labeled mate rials. M ethods a re given in detail fOI' (a) converting t ritium gas to tritium oxide, (b) preparing tritium-labeled li thium borohy dride, and (c) conducting a variety of r eactions in a closed system. Auxilia ry equipment is shown, including water traps, reaction ...
متن کاملPlatelet tagging with tritium labeled diisopropylfluorophosphate.
By EDWARD ADELSON, RICHARD M. KAUFMAN, Cmo BERDEGUEZ, ARNOLD A. LEAR AND JACK J. RHEINGOLD F OR THE PAST 10 years, P32 labeled diisopropylfluorophosphate has been used to tag platelets.”2 This compound attaches to various proteins, during which process it changes from diisopropylfiuorophosphate ( DFP) to diisopropylphosphate (DIP).3 The DIP is irreversibly bound to the protein molecule until th...
متن کاملPurification of tritium-labeled cholera toxin.
Cholera toxin was labeled with tritium by the Wilzbach technique, and highly purified radiolabeled toxin was obtained by Sephadex column chromatography and disc gel electrophoresis. 3H-labeled cholera toxin retained its biological activity and chemical stability and had a specific activity of 405.9 muCi/mumol. The methods utilized in extraction and purification of 3H-labeled toxin may be advant...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry
سال: 1960
ISSN: 0022-4332
DOI: 10.6028/jres.064a.038